Dependence of molecular order in model membranes on the position of the double-bond: a deuterium NMR study (bibtex)
by Curtis Bausmer
Abstract:
Model membrane systems made up of mono-unsaturated phospholipids exhibit a dramatic dependence of their thermodynamic properties in the position of the double bond within the fatty acid chains of the phospholipid molecules. In particular, a minimum is observed in both the structural (Lalpha - HII) phase transition temperature (Epand, E. (1996)) and in the chain-melting (gel to liquid crystalline) phase transition temperature, when the double bond is at carbon 9 of an 18-carbon long fatty acid chain. These results were obtained using x-ray and differential calorimetry data. This report investigates the effects of shifting the double-bond position on the molecular order within the phospholipid molecules, as observed by Deuterium NMR. The initial study showed, somewhat surprisingly, that the effect is not significant in the HII phase. The limitations of obtaining order parameter profiles via a perdeuterated alcohol probe precluded a further investigation in the La phase.
Reference:
Curtis Bausmer, "Dependence of molecular order in model membranes on the position of the double-bond: a deuterium NMR study", 1996.
Bibtex Entry:
@bachelorsthesis{1996B,
  title={Dependence of molecular order in model membranes on the position of the double-bond: a deuterium NMR study},
  author={Curtis Bausmer},
  month={May},
  year={1996},
  abstract={Model membrane systems made up of mono-unsaturated phospholipids
exhibit a dramatic dependence of their thermodynamic properties in the position
of the double bond within the fatty acid chains of the phospholipid
molecules. In particular, a minimum is observed in both the structural (L<sub>alpha</sub> -
H<sub>II</sub>) phase transition temperature (Epand, E. (1996)) and in the
chain-melting (gel to liquid crystalline) phase transition temperature, when the
double bond is at carbon 9 of an 18-carbon long fatty acid chain.  These
results were obtained using x-ray and differential calorimetry data.
This report investigates the effects of shifting the double-bond
position on the molecular order within the phospholipid molecules, as observed
by Deuterium NMR.  The initial study showed, somewhat surprisingly, that
the effect is not significant in the H<sub>II</sub> phase.  The limitations of
obtaining order parameter profiles via a perdeuterated alcohol probe precluded
a further investigation in the La phase.},
  note={Supervised by E. Sternin}
}
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